کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5896683 1568729 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High serum CXCL10 in Rickettsia conorii infection is endothelial cell mediated subsequent to whole blood activation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
High serum CXCL10 in Rickettsia conorii infection is endothelial cell mediated subsequent to whole blood activation
چکیده انگلیسی


- High levels of CXCL10 were observed in patients with R. conorii infection.
- The CXCL10 levels decreased markedly after recovery of the patients.
- R. conorii induced a marked CXCL10 increase in a blood/endothelial co-culture model.
- Plasma from R. conorii-exposed blood induced marked CXCL10 release from endothelium.

BackgroundThe pathophysiological hallmark of Rickettsia conorii (R. conorii) infection comprises infection of endothelial cells with perivascular infiltration of T-cells and macrophages. Although interferon (IFN)-γ-induced protein 10 (IP-10)/CXCL10 is induced during vascular inflammation, data on CXCL10 in R. conorii infection is scarce.MethodsSerum CXCL10 was analyzed in two cohorts of southern European patients with R. conorii infection using multiplex cytokine assays. The mechanism of R. conorii-induced CXCL10 release was examined ex vivo using human whole blood interacting with endothelial cells.Results(i) At admission, R. conorii infected patients had excessively increased CXCL10 levels, similar in the Italian (n = 32, ∼56-fold increase vs controls) and the Spanish cohort (n = 38, ∼68-fold increase vs controls), followed by a marked decrease after recovery. The massive CXCL10 increase was selective since it was not accompanied with similar changes in other cytokines. (ii) Heat-inactivated R. conorii induced a marked CXCL10 increase when whole blood and endothelial cells were co-cultured. Even plasma obtained from R. conorii-exposed whole blood induced a marked CXCL10 release from endothelial cells, comparable to the levels found in serum of R. conorii-infected patients. Bacteria alone did not induce CXCL10 production in endothelial cells, macrophages or smooth muscle cells.ConclusionsWe show a massive and selective serum CXCL10 response in R. conorii-infected patients, likely reflecting release from infected endothelial cells characterized by infiltrating T cells and monocytes. The CXCL10 response could contribute to T-cell infiltration within the infected organ, but the pathologic consequences of CXCL10 in clinical R. conorii infection remain to be defined.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 83, July 2016, Pages 269-274
نویسندگان
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