کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5897364 1155269 2013 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short CommunicationLeucine-rich glioma inactivated 3 associates negatively with adiponectin
ترجمه فارسی عنوان
ارتباط کوتاه ارتباطی با گلیوم غنی از لیکوین 3 عضوی منفی را با آدیپونکتین غیرفعال کرده است
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
چکیده انگلیسی


- We found that adiponectin was increased in adipose tissues and plasma of LGI3 knockout mice.
- LGI3 was upregulated whereas adiponectin was downregulated in high fat diet-fed obese mice.
- Knockdown of LGI3 increased adiponectin and treatment with LGI3 protein decreased adiponectin in 3T3-L1 cells.
- We propose that LGI3 may be a pro-inflammatory adipokine candidate that negatively regulates adiponectin.

Leucine-rich glioma inactivated 3 (LGI3) is a secreted protein member of LGI/epitempin family. We previously reported that LGI3 was expressed in adipose tissues and suppressed adipogenesis through its receptor, ADAM23. We proposed that LGI3 may be a candidate adipokine with pro-inflammatory activity. To investigate the role of LGI3 in adipose tissues, we analyzed cytokine profile in LGI3 knockout mice. Protein array analysis showed that adiponectin was significantly increased in adipose tissues and plasma of LGI3 knockout mice. SiRNA-mediated knockdown of LGI3 increased adiponectin in 3T3-L1 preadipocytes. Treatment of differentiating 3T3-L1 cells with LGI3 protein decreased adiponectin in a dose-dependent manner. High fat diet (HFD)-fed mice showed expression of LGI3 in adipose tissue macrophages in addition to adipocytes that expressed LGI3 in both normal chow-fed and HFD-fed mice. The 60-kDa LGI3 was selectively increased in adipose tissues of HFD mice in which adiponectin was downregulated. Taken together, these results suggested that LGI3 may participate in adipose tissue homeostasis by negatively regulating adiponectin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 62, Issue 2, May 2013, Pages 206-209
نویسندگان
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