کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5901094 1568898 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The osmoregulatory effects of rearing Mozambique tilapia in a tidally changing salinity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
The osmoregulatory effects of rearing Mozambique tilapia in a tidally changing salinity
چکیده انگلیسی


- Tilapia were reared in a salinity that changed between FW and SW every 6 h.
- Plasma prolactin does not correlate with plasma osmolality in tidally reared tilapia.
- Expression of ion transporters in tidally reared fish is similar to that of SW fish.
- Ionocytes of tidally reared tilapia are similar to those of SW-acclimated tilapia.

The native distribution of Mozambique tilapia, Oreochromis mossambicus, is characterized by estuarine areas subject to salinity variations between fresh water (FW) and seawater (SW) with tidal frequency. Osmoregulation in the face of changing environmental salinity is largely mediated through the neuroendocrine system and involves the activation of ion uptake and extrusion mechanisms in osmoregulatory tissues. We compared plasma osmolality, plasma prolactin (PRL), pituitary PRL mRNA, and mRNA of branchial ion pumps, transporters, channels, and PRL receptors in tilapia reared in FW, SW, brackish water (BW) and in tidally-changing salinity, which varied between FW (TF) and SW (TS) every 6 h. Plasma PRL was higher in FW tilapia than in SW, BW, TF, and TS tilapia. Unlike tilapia reared in FW or SW, fish in salinities that varied tidally showed no correlation between plasma osmolality and PRL. In FW fish, gene expression of PRL receptor 1 (PRLR1), Na+/Cl− cotransporter (NCC), aquaporin 3 (AQP3) and two isoforms of Na+/K+-ATPase (NKA α1a and NKA α1b) was higher than that of SW, BW or tidally-changing salinity fish. Gene expression of the Na+/K+/2Cl− cotransporter (NKCC1a), and the cystic fibrosis transmembrane conductance regulator (CFTR) were higher in fish in SW, BW or a tidally-changing salinity than in FW fish. Immunocytochemistry revealed that ionocytes of fish in tidally-changing salinities resemble ionocytes of SW fish. This study indicated that tilapia reared in a tidally-changing salinity can compensate for large changes in external osmolality while maintaining osmoregulatory parameters within a narrow range closer to that observed in SW-acclimated fish.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 207, 1 October 2014, Pages 94-102
نویسندگان
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