Investigating patterns in the spermiation response of eight Australian frogs administered human chorionic gonadotropin (hCG) and luteinizing hormone-releasing hormone (LHRHa)

► Neither hormone is consistently more effective at inducing spermiation across species. ► All five species from the family Myobatrachidae released more spermatozoa in response to LHRHa. ► All three species from the family Lymnodynastidae released more spermatozoa in response to hCG. ► These results provide preliminary evidence that hCG and LHRHa efficacy in anurans may be predicted by phylogeny.