Ghrelin-induced growth hormone release from goldfish pituitary cells is nitric oxide dependent

► 1st demonstration in any teleost: ghrelin direct stimulation of GH release involves nitric oxide (NO) signalling. ► 1st demonstration in any model system: direct ghrelin stimulation of GH release is NOS isoform specific. ► Implicates differential use of NOS isoforms in the multifactorial neuroendocrine control of GH release.