MMP-2 and MMP-9 localization and activity in the female prostate during estrous cycle

► The estrus was the phase of greater activity of MMP-2 and -9 in the female prostate. ► The activity of MMP-2 and -9 was very weak in diestrus I and II. ► Estradiol and progesterone regulate the activity of MMP-2 and -9 in female prostate. ► Activities of MMP-2 and -9 in female prostate were up to 12 times higher than male.