Immunocytochemical study of rhodopsin-containing putative encephalic photoreceptors in house sparrow, Passer domesticus

In seasonally breeding birds, encephalic photoreceptors (EPs) play an important role in regulating photoperiodic gonadal responses. Multiple photopigments have been suggested as the putative EPs, including rhodopsin, melanopsin, VA opsin and the cryptochromes. As for rhodopsin, two potential brain sites, the lateral septum (SL) and the infundibulum (INF) have been reported to co-express rhodopsin immunoreactivity (rhodopsin-ir) with vasoactive intestinal polypeptide immunoreactivity (VIP-ir) in groups of cerebrospinal fluid-contacting (CSF) cells, hypothesized to be the EPs for gonadal responses. In order to confirm the presence of rhodopsin in seasonally breeding birds and examine whether these EPs show daily change as do the photopigments in the retina and pineal gland, the present study immunocytochemically investigated: (1) the presence of rhodopsin expression in the deep brain of the house sparrow, Passer domesticus maintained in short days, and (2) rhythmic expression of rhodopsin and VIP in both SL and INF at Zeitgeber time (ZT) 1 and ZT 17 in house sparrows. Rhodopsin-ir and VIP-ir were observed in both areas of sparrow brains as previously described in other avian species but with a novel rod-like rhodopsin-ir cell type in the INF and novel expression of rhodopsin-ir fiber close to the preoptic area. Daily changes of rhodopsin-ir and VIP-ir cell number were revealed in the INF, but not in the SL. More rhodopsin-ir and fewer VIP-ir cells were found at ZT 17 than at ZT 1. In the median eminence, rhodopsin-ir fibers were only observed at ZT 1, and the relative optic density (ROD) of VIP-ir fibers was higher at ZT 1 than ZT 17. The results indicate daily changes of EPs in the IN and ME, suggesting a role of EPs in the orchestration of photoperiodic gonadal recrudesence.

Research highlights► Rhodopsin immunoreactivity was identified in the SL and INF in house sparrow. ► Both CSF-contacting and novel rod-like neurons were identified in the INF. ► Daily changes of rhodopsin-ir and VIP-ir were found in the INF.