کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905128 | 1159836 | 2016 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
PacBio SMRT assembly of a complex multi-replicon genome reveals chlorocatechol degradative operon in a region of genome plasticity
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کلمات کلیدی
PacbioCBALCBBurkholderiaSMRT3-Chlorobenzoate - 3-کلروبنزواتInsertion sequence - توالی درجNext generation sequencing - توالی نسل بعدیRIT - سوار شدنPacific Biosciences - علوم بیولوژیکی اقیانوس آرامMobile Elements - عناصر موبایلGenome assembly - مونتاژ ژنومPlasmid - پلاسمیدchlorocatechol - کلرکاکتچولIce - یخ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: PacBio SMRT assembly of a complex multi-replicon genome reveals chlorocatechol degradative operon in a region of genome plasticity PacBio SMRT assembly of a complex multi-replicon genome reveals chlorocatechol degradative operon in a region of genome plasticity](/preview/png/5905128.png)
چکیده انگلیسی
We have sequenced a Burkholderia genome that contains multiple replicons and large repetitive elements that would make it inherently difficult to assemble by short read sequencing technologies. We illustrate how the integrated long read correction algorithms implemented through the PacBio Single Molecule Real-Time (SMRT) sequencing technology successfully provided a de novo assembly that is a reasonable estimate of both the gene content and genome organization without making any further modifications. This assembly is comparable to related organisms assembled by more labour intensive methods. Our assembled genome revealed regions of genome plasticity for further investigation, one of which harbours a chlorocatechol degradative operon highly homologous to those previously identified on globally ubiquitous plasmids. In an ideal world, this assembly would still require experimental validation to confirm gene order and copy number of repeated elements. However, we submit that particularly in instances where a polished genome is not the primary goal of the sequencing project, PacBio SMRT sequencing provides a financially viable option for generating a biologically relevant genome estimate that can be utilized by other researchers for comparative studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 586, Issue 2, 25 July 2016, Pages 239-247
Journal: Gene - Volume 586, Issue 2, 25 July 2016, Pages 239-247
نویسندگان
N. Ricker, S.Y. Shen, J. Goordial, S. Jin, R.R. Fulthorpe,