|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5905144||1159847||2016||5 صفحه PDF||سفارش دهید||دانلود رایگان|
- To investigate the effect of three transcription regulators, ABN (Ascl1, Brn2 and Ngn2) on learning and memory loss in a mouse model of AD.
Alzheimer's disease (AD) is an irreversible and progressive neurodegenerative disorder with observable memory impairment. The present study was performed to evaluate the beneficial effects of lentiviral vector-mediated overexpression of a combination of three transcription regulators, ABN (Ascl1, Brn2 and Ngn2), on learning and memory loss in a mouse model of AD. The AD model was established by injecting AÎ²1-42 bilaterally into the mouse hippocampus. Lentiviral ABN was delivered bilaterally into the hippocampus of mice. Animals injected with LV-ABN showed significantly improved spatial learning and memory in the water maze test. Additionally, antibody array analysis indicated that intrahippocampal LV-ABN delivery significantly altered the expression levels of some proteins that were identified as inflammatory factors or neuroprotective and growth factors. In conclusion, our data suggest that LV-ABN delivery can ameliorate spatial learning and memory impairment in an AD mouse model, and the beneficial effect of ABN gene treatment could be linked to inhibition of the neuroinflammatory response and enhancement of neuroprotection and neurogenesis. Thus, these findings indicate that lentiviral ABN gene delivery has potential therapeutic applications for AD.
Journal: Gene - Volume 587, Issue 1, 1 August 2016, Pages 59-63