کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905551 | 1159908 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of zma-miR169 miRNAs and their target ZmNF-YA genes in response to abiotic stress in maize leaves
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کلمات کلیدی
N. benthamianaNicotiana benthamianancRNApiRNANF-YAnoncoding RNA - RNA غیر CodingAbiotic stress - استرس بی روحExpression pattern - الگوی بیانTransient expression - بیان گذراRISC - خطرZea mays - ذرت RNA-Induced Silencing Complex - مجتمع خاموش کننده RNA inducedMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNApiwi-interacting RNA - کیوی-تعامل RNA
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
The miR169 miRNA family is highly conserved in plants. Its members regulate the expression of genes encoding the universal transcription factor subunit NUCLEAR FACTOR-Y subunit A (NF-YA) via transcript cleavage. NF-YA regulates gene expression by binding the CCAAT box sequence in target promoters. The miR169/NF-YA module plays a critical role during plant development and in plant responses to abiotic stress. We characterized the secondary structures of maize pre-miR169 miRNAs and predicted their potential gene targets. Coexpression of zma-miR169 and ZmNF-YA in Nicotiana benthamiana demonstrated that mutations in or deletion of target sites abolished regulation by zma-miR169. Maize seedlings were subjected to short-term (0-48Â h) and long-term (15Â days) drought, abscisic acid (ABA), or salt stress. Long-term exposure to PEG (drought stress) or NaCl (salt stress) repressed seedling growth. We investigated the expression patterns of zma-miR169s and their target ZmNF-YA genes in maize leaves and found diverse changes in expression in response to the three stress treatments. The expression of most zma-miR169 genes was downregulated by PEG and upregulated by ABA. In response to salt stress, zma-miR169 genes were upregulated initially and subsequently downregulated. Most ZmNF-YA genes were upregulated during the short term and downregulated by 15Â days in response to the three stress treatments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 178-185
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 178-185
نویسندگان
Mingda Luan, Miaoyun Xu, Yunming Lu, Lan Zhang, Yunliu Fan, Lei Wang,