کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905593 | 1159908 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genome-wide identification and expression analysis of the polyamine oxidase gene family in sweet orange (Citrus sinensis)
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کلمات کلیدی
qPCRCuAOCopper-containing amine oxidasePolyamine catabolismPCDSPMORFPAOSPDflavin adenine dinucleotidequantitative real-time RT-PCR - RT-PCR زمان واقعی کمAbiotic stress - استرس بی روحSpermine - اسپرمSpermidine - اسپرمیدینFAD - بدGene expression - بیان ژنopen reading frame - قاب خواندن بازPUT - قرار دادنputrescine - قرار دادنCitrus sinensis - مرکبات سینسیسProgrammed cell death - مرگ برنامهریزی شده یاختهHypersensitivity reaction - واکنش بیش از حد حساسیتPolyamine - پلی آمینpolyamine oxidase - پلیامین اکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Polyamine oxidases (PAOs) are FAD-dependent enzymes associated with polyamine catabolism. In plants, increasing evidences support that PAO genes play essential roles in abiotic and biotic stresses response. In this study, six putative PAO genes (CsPAO1-CsPAO6) were unraveled in sweet orange (Citrus sinensis) using the released citrus genome sequences. A total of 203 putative cis-regulatory elements involved in hormone and stress response were predicted in 1.5-kb promoter regions at the upstream of CsPAOs. The CsPAOs can be divided into four major groups, with similar organizations with their counterparts of Arabidopsis thaliana. Transcripts of CsPAOs were detected in leaf, stem, cotyledon, and root, with the highest levels detected in the roots. The CsPAOs displayed various responses to exogenous treatments with polyamines and ABA and were differentially altered by abiotic stresses, including cold, salt, and mannitol. Overexpression of CsPAO3 in tobacco demonstrated that spermidine and spermine were decreased in the transgenic line, while putrescine was significantly enhanced, implying a potential role of this gene in polyamine back conversion. These data provide valuable knowledge for understanding the roles of the PAO genes in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 421-429
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 421-429
نویسندگان
Wei Wang, Ji-Hong Liu,