کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5905641 1159922 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Robust multi-type plasmid modifications based on isothermal in vitro recombination
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Robust multi-type plasmid modifications based on isothermal in vitro recombination
چکیده انگلیسی
A robust strategy for multi-type plasmid modifications is developed based on the isothermal in vitro recombination technology, by which any combination of the sequence modifications can be efficiently achieved in plasmids at any desired position in a seamless manner. As an example, we showed that a plasmid modification with insertion of a GFP gene, deletion of a 623-bp fragment, and substitution of an ampicillin resistance gene by a kanamycin resistance gene was accomplished simultaneously by this method. Therefore, the isothermal in vitro recombination-based multi-type plasmid modification strategy is a useful approach for broad application prospects in molecular biology studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 548, Issue 1, 10 September 2014, Pages 39-42
نویسندگان
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