کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905915 | 1159943 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera
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کلمات کلیدی
GFPDRE-binding proteinABA-responsive elementBroussonetia papyriferaDREBDRECBFCaMVABAAP2/ERF - AP2 / ERFabscisic acid - اسید آبسزیکABRE - باز کردنrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدC-repeat binding factor - عامل اتصال C تکرارdehydration-responsive element - عنصر واکنش دهنده کم آبیRace - مسابقهcauliflower mosaic virus - ویروس موزائیک گل کلمgreen fluorescent protein - پروتئین فلورسنت سبزWoody plant - گیاه وودی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The dehydration-responsive element binding proteins (DREBs) are important transcription factors in the regulation of plant responses to abiotic stresses. In this study, BpDREB2, an AP2/DREB-type transcription factor gene, was cloned from a woody plant, Broussonetia papyrifera by RACE-PCR. Sequence analyses revealed that BpDREB2 protein has three characteristic domains, including an AP2/EREBP, a nuclear localization signal and an acidic activation domain. Yeast one-hybrid assays showed that BpDREB2 protein specifically binds to the DRE sequence and activates the expression of reporter genes in yeast. These results suggested that BpDREB2 protein could function as a transcription factor of DREB family. The expression of BpDREB2 gene was remarkably induced by dehydration and high-salt treatments, but no significant change was observed under ABA or low-temperature conditions. Importantly, transgenic expression of BpDREB2 gene in Arabidopsis significantly enhanced its tolerance to salt and freezing without causing growth retardation. Taken together, these results suggested that BpDREB2 is a novel member of the AP2/EREBP trans-acting factor family which could enhance salt stress tolerance of plants and has the potential application in the improvement of crops and economical tree species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 535, Issue 2, 10 February 2014, Pages 140-149
Journal: Gene - Volume 535, Issue 2, 10 February 2014, Pages 140-149
نویسندگان
Jingwen Sun, Xianjun Peng, Weihong Fan, Mingjuan Tang, Jie Liu, Shihua Shen,