کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905945 | 1159943 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression profiling of the sex-related gene Dmrt1 in adults of the Lusitanian toadfish Halobatrachus didactylus (Bloch and Schneider, 1801)
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کلمات کلیدی
phosphate buffered saline with Tween 20qPCRmRNAORFDMRT1ppmISHPBSTDEPCcDNA - cDNADNA complementary to RNA - DNA مکمل RNAIn situ hybridization - Hybridization در محلAlternative polyadenylation - polyadenylation جایگزینmessenger RNA - RNA messengerSpermatogenesis - اسپرماتوژنزExpression pattern - الگوی بیانOogenesis - اووژنزImmunochemistry - ایمونوشیمیIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدbase pairs - جفت پایهopen reading frame - قاب خواندن بازparts per million - قطعات در میلیونRace - مسابقهUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهquantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیAPA - چی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Doublesex and mab-3 related transcription factor 1 (Dmrt1) gene is a widely conserved gene involved in sex determination and differentiation across phyla. To gain insights on Dmrt1 implication for fish gonad cell differentiation and gametogenesis development, its mRNA was isolated from testis and ovary from the Lusitanian toadfish (Halobatrachus didactylus). The cDNA from Dmrt1 was synthesized and cloned, whereas its quantitative and qualitative gene expression, as well as its protein immunolocalization, were analyzed. A main product of 1.38Â kb, which encodes a protein of 295 aa, was reported, but other minority Dmrt1 products were also identified by RACE-PCR. This gene is predominantly expressed in testis (about 20 times more than in other organs or tissues), specially in spermatogonia, spermatocytes and spermatids, as well as in somatic Sertoli cells, indicating that Dmrt1 plays an important role in spermatogenesis. Although Dmrt1 transcripts also seem to be involved in oogenesis development, and it cannot be excluded that toadfish Dmrt1 could be functionally involved in other processes not related to sex.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 535, Issue 2, 10 February 2014, Pages 255-265
Journal: Gene - Volume 535, Issue 2, 10 February 2014, Pages 255-265
نویسندگان
MarÃa Ãbeda-Manzanaro, Manuel A. Merlo, Juan B. Ortiz-Delgado, Laureana Rebordinos, Carmen Sarasquete,