کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5906165 | 1159958 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dehydration mediated microRNA response in the African clawed frog Xenopus laevis
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کلمات کلیدی
PI3KSAPEJnkp53MFImiRISCMedian fluorescent intensityERKHIF-1αSLCAMPKc-Jun N-terminal kinases - C-Jun N-terminal kinasesMAPK - MAPKNon-coding RNA - RNA غیرکد کننده، RNA غیرترجمه شوندهMetabolic rate depression - افسردگی متابولیکsolute carrier - حامل رقیبKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوAmphibian - دوزیستانhypoxia inducible factor-1α - عامل القایی هیپوکسی 1αphosphatase and tensin homolog - فسفاتاز و تنسین همولوگphosphoinositide 3-kinase - فسفینوزیتید 3-کینازLuminex - لومینکسtumor protein 53 - پروتئین تومور 53mitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استadenosine monophosphate-activated protein kinase - پروتئین کیناز فعال شده با آدنوزین مونوفسفاتPten - ژن PTENextracellular signal-regulated kinases - کیناز های تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Exposure to various environmental stresses induces metabolic rate depression in many animal species, an adaptation that conserves energy until the environment is again conducive to normal life. The African clawed frog, Xenopus laevis, is periodically subjected to arid summers in South Africa, and utilizes entry into the hypometabolic state of estivation as a mechanism of long term survival. During estivation, frogs must typically deal with substantial dehydration as their ponds dry out and X. laevis can endure > 30% loss of its body water. We hypothesize that microRNAs play a vital role in establishing a reversible hypometabolic state and responding to dehydration stress that is associated with amphibian estivation. The present study analyzes the effects of whole body dehydration on microRNA expression in three tissues of X. laevis. Compared to controls, levels of miR-1, miR-125b, and miR-16-1 decreased to 37 ± 6, 64 ± 8, and 80 ± 4% of control levels during dehydration in liver. By contrast, miR-210, miR-34a and miR-21 were significantly elevated by 3.05 ± 0.45, 2.11 ± 0.08, and 1.36 ± 0.05-fold, respectively, in the liver. In kidney tissue, miR-29b, miR-21, and miR-203 were elevated by 1.40 ± 0.09, 1.31 ± 0.05, and 2.17 ± 0.31-fold, respectively, in response to dehydration whereas miR-203 and miR-34a were elevated in ventral skin by 1.35 ± 0.05 and 1.74 ± 0.12-fold, respectively. Bioinformatic analysis of the differentially expressed microRNAs suggests that these are mainly involved in two processes: (1) expression of solute carrier proteins, and (2) regulation of mitogen-activated protein kinase signaling. This study is the first report that shows a tissue specific mode of microRNA expression during amphibian dehydration, providing evidence for microRNAs as crucial regulators of metabolic depression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 529, Issue 2, 25 October 2013, Pages 269-275
Journal: Gene - Volume 529, Issue 2, 25 October 2013, Pages 269-275
نویسندگان
Cheng-Wei Wu, Kyle K. Biggar, Kenneth B. Storey,