کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5906485 | 1159972 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The Fusarium Graminearum virulence factor FGL targets an FKBP12 immunophilin of wheat
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کلمات کلیدی
FKBPFGLeffector-triggered susceptibilityLRRPVXNBSFKBP12FHBETsBiFCDeoxynivalenolERGETI - اتیEffector-triggered immunity - ایمنی عامل اثرImmunophilin - ایمونوفیلینleucine-rich repeat - تکرار غنی از لوسینDON - دونnucleotide-binding site - سایت پیوند نوکلئوتیدیFusarium head blight - سر Fusarium سرگیجه داردHypersensitive reaction - واکنش حساسPotato virus X - ویروس سیب زمینی XFK506-binding protein - پروتئین اتصال دهنده FK506
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Wheat scab, caused by the fungal pathogen Fusarium graminearum is a devastating disease worldwide. Despite an extensive and coordinated effort to investigate this pathosystem, little progress has been made to understand the molecular basis of host-pathogen interactions, for example how the pathogen causes disease in plant. Recently, a secreted lipase (FGL1) has been identified from the fungus and shown to be an important virulence factor; however, the intrinsic function of FGL1 in plant is unknown. Here, we report the identification of the molecular components that may possibly be involved in the FGL virulence pathway using yeast two hybrid system. FGL gene was amplified from a local virulent strain (F15) and shown to be 99.5% identical to the original published FGL at the amino acid level. We showed that transient expression of this FGL gene by Agroinfiltration in tobacco leaves causes cell death further implicating the role of FGL in virulence. To identify FGL initial physical target in plant, we screened two wheat cDNA libraries using the FGL protein as the bait. From both libraries, a small FKBP-type immunophilin protein, designated wFKBP12, was found to physically interact with FGL. The direct interaction of FGL with wFKBP12 was confirmed in living onion epidermal cells by biomolecular fluorescence complementation (BiFC) assay. To investigate further, we then used wFKBP12 protein as bait and identified an elicitor-responsive protein that contains a potential Ca2Â + binding domain. Semi-quantitative PCR showed that this elicitor-responsive gene is down-regulated during the F. graminearum infection suggesting that this protein may be an important component in FGL virulence pathway. This work serves as an initial step to reveal how fungal lipases act as a general virulence factor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 525, Issue 1, 1 August 2013, Pages 77-83
Journal: Gene - Volume 525, Issue 1, 1 August 2013, Pages 77-83
نویسندگان
Xiao-Wei Niu, Zi-Yang Zheng, Yi-Gao Feng, Wang-Zhen Guo, Xin-Yu Wang,