کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5906500 | 1159972 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The extrapituitary prolactin promoter polymorphism is associated with rheumatoid arthritis and anti-CCP antibodies in Mexican population
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کلمات کلیدی
DMARDshPRLanti-CCPDAS-28NSAIDShsCRPPRLESRRheumatoid arthritis - آرتریتروماتوئیدAnti-CCP antibodies - آنتی بادی های ضد CCPcontrol subjects - افراد کنترل شدهdisease modifying anti-rheumatic drugs - بیماری های ضد روماتیسمی را اصلاح می کندnon steroidal anti-inflammatory drugs - داروهای ضد التهابی غیر استروئیدerythrocyte sedimentation rate - سرعت رسوب گلبول قرمزrheumatoid factor - عامل روماتوئیدDisease activity score 28 - نمره فعالیت بیماری 28Hyperprolactinaemia - هیپرپرولاکتینمیProlactin - پرولاکتین Single nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیAnti-cyclic citrullinated peptide - پپتید سیتروالین ضد سیکلSNP - چندریختی تک-نوکلئوتید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Prolactin (PRL) is a hormone-cytokine that has been involved in autoimmunity due to its immunoregulatory and lymphoproliferative effects. It is produced by various extrapituitary sites including immune cells, under control of a superdistal promoter that contains a single nucleotide polymorphism â 1149 G/T previously associated with rheumatoid arthritis (RA) susceptibility in European population. The aim of this study was to investigate the association of the extrapituitary PRL â 1149 G/T promoter polymorphism with clinical parameters, clinical activity and disability indices in RA patients from Western Mexico and to analyze the PRL mRNA expression according to the PRL â 1149 G/T promoter polymorphism in total leucocytes from RA patients and controls. We conducted a case-control study that included 258 RA patients and 333 control subjects (CS). The DNA samples were genotyped using the PCR-RFLP method and the PRL mRNA expression was determined by quantitative real time PCR. PRL serum levels and antibodies to cyclic citrullinated peptides (anti-CCP) were measured with ELISA. We found significant differences in the genotype (p = 0.022) and allelic (p = 0.046) distribution of the polymorphism between RA patients and control subjects. According to the dominant genetic model, there is an association between the T allele (GT + TT genotypes) and decreased RA susceptibility in comparison to the G allele carriers (GG genotype) (OR 0.64, 95% CI 0.45-0.92; p = 0.011). The T allele carriers (GT + TT genotypes) had lower titers of anti-CCP antibodies in comparison to the G allele carriers (GG genotype) (median, 66 U/mL vs. 125 U/mL; p = 0.03). Furthermore, the GG homozygotes had higher PRL mRNA expression in comparison to the GT heterozygotes, and this latter with respect to the TT homozygotes, in both groups (RA: 1 > 0.72 > 0.19; CS: 1 > 0.54 > 0.28). However, PRL serum levels were similar in both groups. Our results suggest that the PRL â 1149 T allele is a genetic marker for decreased RA susceptibility and is associated with lower titers of anti-CCP antibodies in Mexican population. We also suggest influence of genotype upon PRL mRNA expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 525, Issue 1, 1 August 2013, Pages 130-135
Journal: Gene - Volume 525, Issue 1, 1 August 2013, Pages 130-135
نویسندگان
Zyanya Reyes-Castillo, Ana Laura Pereira-Suárez, Claudia Azucena Palafox-Sanchez, Héctor Rangel-Villalobos, Ciro Estrada-Chávez, Edith Oregón-Romero, Luis Ignacio Angel-Chávez, Salvador Muñoz-Barrios, Miriam Ruth Bueno-Topete,