کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5906555 1159974 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Myocardin-related transcription factor-A is a key regulator in retinoic acid-induced neural-like differentiation of adult bone marrow-derived mesenchymal stem cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Myocardin-related transcription factor-A is a key regulator in retinoic acid-induced neural-like differentiation of adult bone marrow-derived mesenchymal stem cells
چکیده انگلیسی


- We reported the effects of MRTF-A in neuronal differentiation of MSCs.
- Loss of function of MRTF-A inhibited upregulation of RA-induced neural markers.
- MRTF-A and RA can synergistically activate transcription of NF-H.

Mesenchymal stem cells (MSCs) have multilineage differentiation potential and can differentiate into neuron cells under appropriate environment in vitro and in vivo. Retinoic acid (RA), a vitamin A derivative, is known to facilitate the neuronal differentiation of MSCs. However, the mechanism by which RA induced MSC differentiation into neuron-like cells is not completely understood. Here, we show that RA can induce neural-like differentiation of bone marrow-derived MSCs, as evidenced by the increase of neuron-specific marker expression and the gradually decreased resting membrane potential. Of note, myocardin-related transcription factor-A (MRTF-A), a major co-activator of serum response factor (SRF), was significantly activated and its nuclear localization was observed during RA-induced neural-like differentiation. MRTF-A is recently reported to function in the development of the nervous system. Our results demonstrated that dominant-negative form of MRTF-A (DN-MRTF-A) or shRNA-MRTF-A strongly inhibited upregulation of neural markers in response to RA. Furthermore, reporter assays with NF-H promoter indicated that RA and MRTF-A can synergistically activate NF-H transcription and enhance the mRNA expression of NF-H. These findings reveal that MRTF-A is a key regulator in all-trans RA-induced neural-like differentiation of bone marrow-derived MSCs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 523, Issue 2, 10 July 2013, Pages 178-186
نویسندگان
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