New yeast/E. coli/Drosophila triple shuttle vectors for efficient generation of Drosophila P element transformation constructs

► We introduce yeast/E. coli/Drosophila triple shuttle vectors for simplified cloning. ► These vectors allow the integration of PCR-derived sequences at any chosen position. ► The vectors allow fast construction of Drosophila P-element transformation vectors. ► We tested the vectors, e.g., to drive nuclear mCherry in transgenic Drosophila.