A new approach to touch down method using betaine as co-solvent for increased specificity and intensity of GC rich gene amplification

► Initial annealing temperature below the primers Tm. ► Amplicon yield could effectively serve for RFLP, blotting and DNA sequencing. ► It is less time taking. ► Economic, requiring lower volumes of reaction mixture. ► Absence of spurious products that impede during RFLP and DNA sequencing.