کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5907652 | 1160132 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of a granulin-like transcript expressed during anoxic exposure and translated during aerobic recovery in a marine gastropod
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کلمات کلیدی
PMSFSDSLittorina littoreadCTPDEPCPVDFdb-cAMPORFphorbol 12-myristate 13-acetatecDNA - cDNADNA complementary to RNA - DNA مکمل RNAPMA - LDC هاROS - ROSEST - استUltraviolet - اشعه فرابنفشOxygen - اکسیژنOxidative stress - تنش اکسیداتیوExpressed Sequence Tag - تکرار اظهار نظرDamage - خسارتdeoxycytidine 5′-triphosphate - دگزوسی سیتیدین 5'-تری فسفاتdiethylpyrocarbonate - دیاتیلپیر کربناتTranscription - رونویسیsodium dodecyl sulfate - سدیم دودسیل سولفاتphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدopen reading frame - قاب خواندن بازHypoxia - هیپوکسیNitrogen gas - گاز نیتروژنReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
A novel transcript encoding a cysteine-rich granulin-like peptide (l-grn) was identified in the hepatopancreas of the marine intertidal gastropod, Littorina littorea, an anoxia-tolerant species. Experimental exposure of snails to anoxia induced a gradual accumulation of l-grn transcripts over time, with expression regulated in vitro through elements responsive to second messengers of protein kinases A, C and G. Translation of this transcript was analyzed by examining l-grn association with ribosomes during normoxia, anoxia, and aerobic recovery. Transcripts of l-grn were associated with polysomes during normoxia, moved into the monosome fractions under anoxia, but shifted back to the polysomal fractions during aerobic recovery. Western blotting confirmed this with a granulin-like protein detected under normoxic conditions, but not during anoxia exposure. A significant increase in the precursor protein and peptide (L-GRN) was observed during the aerobic recovery period. The accumulation of l-grn transcripts during anoxic exposure and subsequent translation following the return to aerobic conditions may be a response to oxidant damage that occurs during re-oxygenation. Overall, the data show that the l-grn gene is anoxia-responsive in this species and may have pro-survival functions during the recovery period.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 410, Issue 1, 29 February 2008, Pages 37-43
Journal: Gene - Volume 410, Issue 1, 29 February 2008, Pages 37-43
نویسندگان
Kevin Larade, Kenneth B. Storey,