Coding region of adiponectin contains a silent intron reactivated by the adjacent intervening sequence of vector

► A sequence in the native cDNA was missing from the transfected ADPN gene. ► The missing sequence has a typical intron configuration. ► A point mutation at the acceptor site (AG → TG) abolished this splicing site. ► The adjacent intervening sequence (IVS) reactivated the silent intron.