Construction of integrative plasmids suitable for genetic modification of industrial strains of Saccharomyces cerevisiae

► Integrative plasmids are reported for transformation of industrial yeast. ► The modular architecture allows easy changes in integration and gene expression. ► Glucose-repressive metabolic marker makes transformation selection easier. ► Multiples integrations in the yeast genome are free of harmful sequences. ► The blue-print phenotype helps to trace the recombinant cells in the environment.