Two replication regions in the pJM1 virulence plasmid of the marine pathogen Vibrio anguillarum

► Two replication regions were identified in the Vibrio anguillarum pJM1 plasmid. ► The copy number of pJM1 is 1-2. ►The ori1 requires plasmid or host proteins for its replication. ► The ori1 region replicates in both Escherichia coli and V. anguillarum. ► The ori2 region only replicates in V. anguillarum.