کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5912960 1161632 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of conjugation rates on solid surfaces
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Determination of conjugation rates on solid surfaces
چکیده انگلیسی

A cytometric method for the estimation of end-point conjugation rates is developed and adapted to surface conjugation. This method improves the through-put of conjugation assays based on replica-plating and results in less noisy experimental data. Although conjugation on solid surfaces deviates from ideal conditions in which cells are continuously mixed, results show that, within the limits of high initial population densities and short mating times, end-point estimates of the conjugation rates are robust measurements. They are independent of the donor/recipient ratios and, to some extent, of the sampling time. Remixing the mating population in the course of a conjugation experiment results in a boost in the frequency of transconjugants.

► A cytometric method to determine surface conjugation rates. ► End-point estimation of conjugation rates adapted to this method. ► Experimental data on transfer rates of plasmid R388 oriT.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 67, Issue 2, March 2012, Pages 174-182
نویسندگان
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