کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5912966 1161632 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Eclipse period of R1 plasmids during downshift from elevated copy number: Nonrandom selection of copies for replication
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Eclipse period of R1 plasmids during downshift from elevated copy number: Nonrandom selection of copies for replication
چکیده انگلیسی

The classical Meselson-Stahl density-shift method was used to study replication of pOU71, a runaway-replication derivative of plasmid R1 in Escherichia coli. The miniplasmid maintained the normal low copy number of R1 during steady growth at 30 °C, but as growth temperatures were raised above 34 °C, the copy number of the plasmid increased to higher levels, and at 42 °C, it replicated without control in a runaway replication mode with lethal consequences for the host. The eclipse periods (minimum time between successive replication of the same DNA) of the plasmid shortened with rising copy numbers at increasing growth temperatures (Olsson et al., 2003). In this work, eclipse periods were measured during downshifts in copy number of pOU71 after it had replicated at 39 and 42 °C, resulting in 7- and 50-fold higher than normal plasmid copy number per cell, respectively. Eclipse periods for plasmid replication, measured during copy number downshift, suggested that plasmid R1, normally selected randomly for replication, showed a bias such that a newly replicated DNA had a higher probability of replication compared to the bulk of the R1 population. However, even the unexpected nonrandom replication followed the copy number kinetics such that every generation, the plasmids underwent the normal inherited number of replication, n, independent of the actual number of plasmid copies in a newborn cell.

► Eclipse period for R1 replication during copy number downshift is negative. ► Random choice of plasmid copies for replication fails with copy number >> “n”. ► Replicated R1 copies are preferably chosen for next round of replication. ► ''+n” rule for holds for replication with non-random choice of plasmid copies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 67, Issue 2, March 2012, Pages 191-198
نویسندگان
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