کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5913940 1162711 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Semi-automated selection of cryo-EM particles in RELION-1.3
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Semi-automated selection of cryo-EM particles in RELION-1.3
چکیده انگلیسی
The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION. At the heart of the procedure lies a fully CTF-corrected template-based picking algorithm, which is supplemented by a fast sorting algorithm and reference-free 2D class averaging to remove false positives. With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection. Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 189, Issue 2, February 2015, Pages 114-122
نویسندگان
,