کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5913948 | 1162711 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of a “silver bullet” to resolve crystal lattice dislocation disorder: A cobalamin complex of Î1-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis
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کلمات کلیدی
GSAflavin adenine dinucleotideP5CP5CDHMADmPDDMBNAD+ - NAD +Δ1-pyrroline-5-carboxylate dehydrogenase - Δ1-پریرولین-5-کربوکسیلات دی هیدروژنازFAD - بدCrystal structure - ساختار کریستالیnicotinamide adenine dinucleotide - نیکوتین آمید adenine dinucleotidemultiwavelength anomalous dispersion - پراکندگی غیرمولد چند ضلعیProtein crystallization - کریستال شدن پروتئین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
The use of small molecules as “silver bullets” that can bind to generate crosslinks between protein molecules has been advanced as a powerful means of enhancing success in protein crystallization (McPherson and Cudney, 2006). We have explored this approach in attempts to overcome an order-disorder phenomenon that complicated the structural analysis of the enzyme Î1-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis (P5CDH, Mtb-PruA). Using the Silver Bullets Bio screen, we obtained new crystal packing using cobalamin as a co-crystallization agent. This crystal form did not display the order-disorder phenomenon previously encountered. Solution of the crystal structure showed that cobalamin molecules are present in the crystal contacts. Although the cobalamin binding probably does not have physiological relevance, it reflects similarities in the nucleotide-binding region of Mtb-PruA, with the nucleotide loop of cobalamin sharing the binding site for the adenine moiety of NAD+.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 189, Issue 2, February 2015, Pages 153-157
Journal: Journal of Structural Biology - Volume 189, Issue 2, February 2015, Pages 153-157
نویسندگان
Thomas Lagautriere, Ghader Bashiri, Edward N. Baker,