کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
591422 1453669 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Experimental Approach Using Covalently Attached Fluorophore for Quantification of Oligonucleotide Immobilization on Gold Nanoparticles
ترجمه فارسی عنوان
رویکرد تجربی با استفاده از فلوئوروفور کوانتومی مرتبط با کوانتومی امولسیون کردن اولیگونوکلئوتید بر روی نانوذرات طلا
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
چکیده انگلیسی

In order to control the process of oligonucleotide immobilization on the surface of citrate-stabilized gold nanoparticles, an experimental approach for evaluation of the amount of immobilized oligonucleotides was proposed. This approach is based on using thiolated oligonucleotides with covalently attached fluorophore Cy3 and on comparison of the oligonucleotides concentration before and after immobilization procedure (initial concentration and concentration of unbound oligonucleotides). Their difference gives an unambiguous amount of immobilized oligonucleotides. Application of the proposed experimental approach showed that the efficiency of oligonucleotide immobilization on the surface of gold nanoparticles is strongly dependent on the ionic strength of the medium, oligonucleotide concentration and the temperature of immobilization. The proposed experimental approach for determination of the level of oligonucleotide immobilization on the surface of gold nanoparticles is simple, fast and does not use any toxic chemicals.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Interface Science Communications - Volume 1, August 2014, Pages 35–38
نویسندگان
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