کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5914335 1162733 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Clathrin-coated vesicles from brain have small payloads: A cryo-electron tomographic study
ترجمه فارسی عنوان
باکتری های کلاچین پوشش داده شده از مغز، اطلاعات کمتری دارند: یک مطالعه توموگرافی کریو الکترون
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
چکیده انگلیسی

Clathrin coats, which stabilize membrane curvature during endocytosis and vesicular trafficking, form highly polymorphic fullerene lattices. We used cryo-electron tomography to visualize coated particles in isolates from bovine brain. The particles range from ∼66 to ∼134 nm in diameter, and only 20% of them (all ⩾80 nm) contain vesicles. The remaining 80% are clathrin “baskets”, presumably artifactual assembly products. Polyhedral models were built for 54 distinct coat geometries. In true coated vesicles (CVs), most vesicles are offset to one side, leaving a crescent of interstitial space between the coat and the membrane for adaptor proteins and other components. The latter densities are fewer on the membrane-proximal side, which may represent the last part of the vesicle to bud off. A small number of densities - presumably cargo proteins - are associated with the interior surface of the vesicles. The clathrin coat, adaptor proteins, and vesicle membrane contribute almost all of the mass of a CV, with most cargoes accounting for only a few percent. The assembly of a CV therefore represents a massive biosynthetic effort to internalize a relatively diminutive payload. Such a high investment may be needed to overcome the resistance of membranes to high curvature.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 184, Issue 1, October 2013, Pages 43-51
نویسندگان
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