کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5914349 1162734 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Particle quality assessment and sorting for automatic and semiautomatic particle-picking techniques
ترجمه فارسی عنوان
ارزیابی کیفیت ذرات و دسته بندی تکنیک های جمع آوری ذرات اتوماتیک و نیمه اتوماتیک
کلمات کلیدی
میکروسکوپ الکترونی، جمع آوری ذرات، فراگیری ماشین، تجزیه و تحلیل ذرات تک
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
چکیده انگلیسی

Three-dimensional reconstruction of biological specimens using electron microscopy by single particle methodologies requires the identification and extraction of the imaged particles from the acquired micrographs. Automatic and semiautomatic particle selection approaches can localize these particles, minimizing the user interaction, but at the cost of selecting a non-negligible number of incorrect particles, which can corrupt the final three-dimensional reconstruction. In this work, we present a novel particle quality assessment and sorting method that can separate most erroneously picked particles from correct ones. The proposed method is based on multivariate statistical analysis of a particle set that has been picked previously using any automatic or manual approach. The new method uses different sets of particle descriptors, which are morphology-based, histogram-based and signal to noise analysis based. We have tested our proposed algorithm with experimental data obtaining very satisfactory results. The algorithm is freely available as a part of the Xmipp 3.0 package [http://xmipp.cnb.csic.es].

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 183, Issue 3, September 2013, Pages 342-353
نویسندگان
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