کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5914375 | 1162734 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (13C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to α-chitin, known to be present in other animals, than to β-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 183, Issue 3, September 2013, Pages 474-483
Journal: Journal of Structural Biology - Volume 183, Issue 3, September 2013, Pages 474-483
نویسندگان
Hermann Ehrlich, Oksana V. Kaluzhnaya, Eike Brunner, Mikhail V. Tsurkan, Alexander Ereskovsky, Micha Ilan, Konstantin R. Tabachnick, Vasilii V. Bazhenov, Silvia Paasch, Martin Kammer, René Born, Allison Stelling, Roberta Galli, Sergei Belikov,