کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5915039 | 1162771 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Automatic identification and clustering of chromosome phenotypes in a genome wide RNAi screen by time-lapse imaging
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کلمات کلیدی
Pattern recognition - بازشناخت الگوTime-lapse imaging - تصویربرداری زمانیClustering - خوشه بندیTime series - سری زمانیhigh throughput screening - غربالگری بالاSupport vector machines - ماشین بردار پشتیبانیMathematical morphology - مورفولوژی ریاضیImage processing - پردازش تصویرMachine learning - یادگیری ماشین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
High-throughput time-lapse microscopy is an excellent way of studying gene function by collecting time-resolved image data of the cellular responses to gene perturbations. With the increase in both data amount and complexity, computational methods capable of dealing with large image data sets are required. While image processing methods have been successfully applied to endpoint assays in the past, the analysis of complex time-resolved read-outs was so far still too immature to be applied on a large-scale. Here, we present a complete computational processing pipeline for such screens. By automatic image processing and machine learning, a quantitative description of phenotypic dynamics is obtained from the raw bitmaps. In order to visualize the resulting phenotypes in their temporal context, we introduce Event Order Maps allowing a concise representation of the major tendencies of causes and consequences of phenotypic classes. In order to cluster the phenotypic kinetics, we propose a novel technique based on trajectory representation of multidimensional time series. We demonstrate the use of these methods applying them on a genome wide RNAi screen by time-lapse microscopy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 170, Issue 1, April 2010, Pages 1-9
Journal: Journal of Structural Biology - Volume 170, Issue 1, April 2010, Pages 1-9
نویسندگان
Thomas Walter, Michael Held, Beate Neumann, Jean-Karim Hériché, Christian Conrad, Rainer Pepperkok, Jan Ellenberg,