کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5915192 1162790 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Achievable resolution from images of biological specimens acquired from a 4k × 4k CCD camera in a 300-kV electron cryomicroscope
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Achievable resolution from images of biological specimens acquired from a 4k × 4k CCD camera in a 300-kV electron cryomicroscope
چکیده انگلیسی
Bacteriorhodopsin and ε 15 bacteriophage were used as biological test specimens to evaluate the potential structural resolution with images captured from a 4k × 4k charge-coupled device (CCD) camera in a 300-kV electron cryomicroscope. The phase residuals computed from the bacteriorhodopsin CCD images taken at 84,000× effective magnification averaged 15.7° out to 5.8-Å resolution relative to Henderson's published values. Using a single-particle reconstruction technique, we obtained an 8.2-Å icosahedral structure of ε 15 bacteriophage with the CCD images collected at an effective magnification of 56,000×. These results demonstrate that it is feasible to retrieve biological structures to a resolution close to 2/3 of the Nyquist frequency from the CCD images recorded in a 300-kV electron cryomicroscope at a moderately high but practically acceptable microscope magnification.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 163, Issue 1, July 2008, Pages 45-52
نویسندگان
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