کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5915252 | 1162799 | 2007 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fast, robust, and accurate determination of transmission electron microscopy contrast transfer function
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Fast, robust, and accurate determination of transmission electron microscopy contrast transfer function Fast, robust, and accurate determination of transmission electron microscopy contrast transfer function](/preview/png/5915252.png)
چکیده انگلیسی
Transmission electron microscopy, as most imaging devices, introduces optical aberrations that in the case of thin specimens are usually modeled in Fourier space by the so-called contrast transfer function (CTF). Accurate determination of the CTF is crucial for its posterior correction. Furthermore, the CTF estimation must be fast and robust if high-throughput three-dimensional electron microscopy (3DEM) studies are to be carried out. In this paper we present a robust algorithm that fits a theoretical CTF model to the power spectrum density (PSD) measured on a specific micrograph or micrograph area. Our algorithm is capable of estimating the envelope of the CTF which is absolutely needed for the correction of the CTF amplitude changes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 160, Issue 2, November 2007, Pages 249-262
Journal: Journal of Structural Biology - Volume 160, Issue 2, November 2007, Pages 249-262
نویسندگان
C.O.S. Sorzano, S. Jonic, R. Núñez-RamÃrez, N. Boisset, J.M. Carazo,