کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5915355 1163282 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short technical reportGeneration of an aquaglyceroporin AQP1 null mutant in Leishmania major
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Short technical reportGeneration of an aquaglyceroporin AQP1 null mutant in Leishmania major
چکیده انگلیسی


- Inactivation of the Leishmania AQP1 gene was difficult.
- Leishmania cells without AQP1 are viable.
- Leishmania AQP1 is involved in cellular volume regulation.
- Leishmania AQP1 null mutants are resistant to antimony.

The Leishmania aquaglyceroporin AQP1 plays an important physiological role in water and uncharged polar solutes transport, volume regulation, osmotaxis, and is a key determinant of antimony resistance. By targeted gene disruption, we generated a Leishmania major promastigote AQP1 null mutant. This required several attempts but a chromosomal null AQP1 mutant was obtained by loss of heterozygosity in the presence of a rescue plasmid encoding AQP1. Growth in the absence of selection led to the loss of the rescuing plasmid, indicating that AQP1 is not essential for Leishmania viability. The AQP1-null mutant was resistant to antimonyl tartrate (SbIII) and arsenite (AsIII) due to a decrease import of these metalloids. It also exhibited alterations in its osmoregulation abilities compared with wild-type cells. This is the first report of the generation of a genetic AQP1 null mutant in Leishmania parasite, confirming its physiological function and role in resistance to antimonials, the therapeutic mainstay against Leishmania.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 201, Issue 2, June 2015, Pages 108-111
نویسندگان
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