کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5915410 1570650 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The single epsin homolog in Giardia lamblia localizes to the ventral disk of trophozoites and is not associated with clathrin membrane coats
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
The single epsin homolog in Giardia lamblia localizes to the ventral disk of trophozoites and is not associated with clathrin membrane coats
چکیده انگلیسی


- The Giardia lamblia genome codes for a putative truncated epsinR homolog.
- The giardial epsin is not involved in membrane trafficking.
- The C-terminal domain targets giardial epsin to the ventral disk organelle.
- The N-terminal ENTH domain binds to phosphatidylinositol 3,4,5-trisphosphate.
- Giardial epsin links the plasma membrane to the ventral disk cytoskeleton.

Epsins serve as recruitment platforms for clathrin membrane coat protein components and induce membrane curvature via their N-terminal homology (ENTH) domain. Unexpectedly, the single ENTH domain protein, a putative epsinR homolog (Glepsin), in the diverged protozoan parasite Giardia lamblia, localizes exclusively to the specialized attachment organelle, the ventral disk (VD). Glepsin binds both to phosphatidylinositol (3,4,5)-trisphosphate phospholipids and the VD cytoskeleton, but lacks canonical domains for interaction with clathrin coat components. This suggests reassignment of giardial epsin function from membrane trafficking to a structural role in linking the plasma membrane to the highly specialized VD during evolution of this genus.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 197, Issues 1–2, October 2014, Pages 24-27
نویسندگان
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