Ribosomal RNA and protein transcripts persist in the cysts of Entamoeba invadens

- We labeled newly-synthesized rRNA during encystations, at different time points with [methyl-3H] methionine.
- The transcript levels of selected r-protein genes were seen during encystations.
- We have shown here that rRNA and r-protein transcription may be coordinately regulated.

In most organisms rDNA transcription ceases under conditions of growth stress. However, we have earlier shown that pre-rRNA accumulates during encystation in Entamoeba invadens. We labeled newly-synthesized rRNA during encystation, with [methyl-3H] methionine in the presence of chitinase to enable uptake of isotope. Incorporation rate reduced after 24 h, and then increased to reach levels comparable with normal cells. The label was rapidly chased to the ribosomal pellet in dividing cells, while at late stages of encystation the ratio of counts going to the pellet dropped 3-fold. The transcript levels of selected ribosomal protein genes also went down initially but went up again at later stages of encystation. This suggested that rRNA and ribosomal protein transcription may be coordinately regulated. Our data shows that encysting E. invadens cells accumulate transcripts of both the RNA and protein components of the ribosome, which may ensure rapid synthesis of new ribosomes when growth resumes.

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