کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5915453 | 1163296 | 2014 | 11 صفحه PDF | دانلود رایگان |
- Comparison of four closely related representative Kinetoplastida.
- Inverted relationship between complex I and II activity.
- Inverted relationship between activities of standard enzymes and G3PDH.
- Confirmed absence of TAO in Crithidia and Leishmania.
Trypanosomatids are unicellular parasites living in a wide range of host environments, which to large extent shaped their mitochondrial energy metabolism, resulting in quite large differences even among closely related flagellates. In a comparative manner, we analyzed the activities and composition of mitochondrial respiratory complexes in four species (Leishmania tarentolae, Crithidia fasciculata, Phytomonas serpens and Trypanosoma brucei), which represent the main model trypanosomatids. Moreover, we measured the activity of mitochondrial glycerol-3-phosphate dehydrogenase, the overall oxygen consumption and the mitochondrial membrane potential in each species. The comparative analysis suggests an inverse relationship between the activities of respiratory complexes I and II, as well as the overall activity of the canonical complexes and glycerol-3-phosphate dehydrogenase. Our comparative analysis shows that mitochondrial functions are highly variable in these versatile parasites
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Journal: Molecular and Biochemical Parasitology - Volume 193, Issue 1, January 2014, Pages 55-65