کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5915479 | 1570654 | 2013 | 5 صفحه PDF | دانلود رایگان |
- We have GFP-tagged the LCCL proteins PbLAP4, PbLAP5 and PbLAP6 in Plasmodium berghei.
- PbLAP4, PbLAP5 and PbLAP6 associate with the crystalloid organelle in ookinetes.
- Translational repression controls expression of the LCCL protein repertoire in gametocytes.
Plasmodium LCCL proteins comprise a family of six proteins that function as a protein complex and have essential roles in sporozoite transmission. In Plasmodium berghei, family members PbLAP1, PbLAP2 and PbLAP3 have been shown to be expressed in gametocytes and, following gametogenesis and fertilization, to be targeted to distinctive multivesicular organelles termed crystalloids that form in the ookinete. Here, we show by GFP-tagging that PbLAP4, PbLAP5 and PbLAP6, like their family members, are associated with the crystalloids. However, in contrast to their family members, protein expression of PbLAP4, PbLAP5 and PbLAP6 was not detected in gametocytes, even though transcription of the corresponding genes is most prominent in the sexual blood stage parasites. These results suggest that translational repression controls expression of the LCCL protein repertoire and, consequently, the temporal function of the protein complex during P. berghei development in the mosquito.
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Journal: Molecular and Biochemical Parasitology - Volume 189, Issues 1â2, May 2013, Pages 38-42