Glu-108 is essential for subunit assembly and dimer stability of d-phosphoglycerate dehydrogenase from Entamoeba histolytica

► Low pH induces changes in functional and structural features of EhPGDH. ► Protonation of acidic amino acid residues at pH 5 leads to subunit dissociation. ► Glu-108 is essential for subunit assembly. ► The E108A mutant existed as monomer even at pH 7. ► The electrostatic interactions play a crucial role in the protein stability.