The activity and hydrogen peroxide sensitivity of the peroxiredoxins from the parasitic nematode Haemonchus contortus

The requirement of aerobic organisms to control damage caused by reactive oxygen species has led to the evolution of the antioxidant systems. Peroxiredoxins are a large family of peroxidases which detoxify hydrogen peroxide at the expense of thiols. The parasitic nematode Haemonchus contortus contains two peroxiredoxins, HcPrx1 a mitochondrial protein and HcPrx2 a cytoplasmic protein. Although both peroxiredoxins contain the conserved eukaryotic motifs ‘GGLG’ and ‘YF’, identified as critical for hydrogen peroxide instability, both were stable to high concentrations of hydrogen peroxide, demonstrating different functions to their mammalian counterparts. H. contortus also contains two thioredoxin reductases and five different thioredoxin-like proteins. The activity of both peroxiredoxins was specific for the thioredoxin system; however, both could also be regenerated by the glutathione system when coupled to the nematode specific thioredoxin HcTrx5. Analysis of homologous genes in Caenorhabditis elegans showed that only CePrx2, which is secreted, was sensitive to the external oxidant hydrogen peroxide. However, both peroxiredoxins KO C. elegans were sensitive to intracellular free radicals and both peroxiredoxins protected DNA from free radical attack. The results demonstrate that the hydrogen peroxide detoxification and the antioxidant activity of the peroxiredoxins are separate activities that are independent of the ‘GGLG’ and ‘YF’ motifs.

H. contortus has two Prxs, a mitochondrial enzyme HcPrx1, and a cytoplasmic enzyme HcPrx2, which is also secreted by the nematode.Figure optionsDownload high-quality image (105 K)Download as PowerPoint slideResearch highlights▶ Two typical 2-Cys peroxiredoxins have been identified in Haemonchus contortus. ▶ The activity of both peroxiredoxins was specific for the thioredoxin system. ▶ Both peroxiredoxins could utilise the glutathione system when coupled with HcTrx5. ▶ Both peroxiredoxins were stable to high concentrations of hydrogen peroxide. ▶ The cytoplasmic and secreted HcPrx2 protects against external oxidant attack.