Hydrogenosome-localization of arginine deiminase in Trichomonas vaginalis

▶ Arginine deiminase (ADI) localizes to T. vaginalis hydrogenosomes. ▶ Three ADI sequences were identified in the T. vaginalis genome. ▶ All ADI genes had mitochondrial targetting sequences. ▶ Proteinase k treated ADI-1 transfected hydrogenosomes was visible by immunoblot. ▶ Proteinase k treatment of lysed ADI-1 transfected hydrogenosmes had no immunoblot signal.