کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5915917 | 1163340 | 2009 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of core components of the exon junction complex in trypanosomes
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of core components of the exon junction complex in trypanosomes Identification of core components of the exon junction complex in trypanosomes](/preview/png/5915917.png)
چکیده انگلیسی
In animal cells, the exon junction complex (EJC) is deposited onto mRNAs during the second step of splicing, 20-24Â nt upstream of the exon-exon junction. The EJC core contains four proteins: Mago, Y14, eIF4AIII and Btz. In trypanosomes, cis-splicing is very rare but all mRNAs are subject to 5â² trans-splicing of a 39-nt RNA sequence. Here we show that trypanosomes have a conserved Mago and a divergent Y14 protein, but we were unable to identify a Btz orthologue. We demonstrate that Mago and Y14 form a stable heterodimer using yeast two hybrid analyses. We also show that this complex co-purifies in vivo in trypanosomes with a protein containing an NTF2 domain, typically involved in mRNA transport.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 166, Issue 2, August 2009, Pages 190-193
Journal: Molecular and Biochemical Parasitology - Volume 166, Issue 2, August 2009, Pages 190-193
نویسندگان
Natalia Bercovich, Mariano J. Levin, Christine Clayton, Martin P. Vazquez,