کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5916887 | 1163761 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
NFATc2 (NFAT1) assists BCR-mediated anergy in anti-insulin B cells
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کلمات کلیدی
HBSSNonobese diabetic mouseT1DBCRMFINFATNODFBSinsulin - انسولینhen egg lysozyme - تخم مرغ لیزوزیمTolerance - تلرانس autoimmunity - خودایمنیType 1 diabetes - دیابت نوع۱fetal bovine serum - سرم جنین گاوSignaling - سیگنالینگfollicular - فولیکولارB cell - لنفوسیت بیmarginal zone - منطقه حاشیه ایmean fluorescence intensity - میانگین شدت فلورسانسAntibody - پادتَن یا آنتیبادیHEL - کاملB cell receptor - گیرنده سلول B
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: NFATc2 (NFAT1) assists BCR-mediated anergy in anti-insulin B cells NFATc2 (NFAT1) assists BCR-mediated anergy in anti-insulin B cells](/preview/png/5916887.png)
چکیده انگلیسی
NFAT transcription factors play critical roles in both the activation and repression of T and B lymphocyte responses. To understand the role of NFATc2 (NFAT1) in the maintenance of tolerance for anti-insulin B cells, functionally inactive NFATc2 (NFATc2â/â) was introduced into C57BL/6 mice that harbor anergic anti-insulin 125Tg B cells. The production and peripheral maturation of anti-insulin B cells into follicular and marginal zone subsets was not altered by the absence of functional NFATc2. Surface B cell receptor expression levels, important for tonic signaling and altered by anergy, were not altered in any spleen B cell subset. The levels of anti-insulin antibodies were not different in 125Tg/B6/NFATc2â/â mice and the anti-insulin response remained silenced following T cell dependent immunization. However, studies addressing in vitro proliferation reveal the anergic state of 125Tg B cells is relieved in 125Tg/B6/NFATc2â/â B cells in response to BCR stimulation. In contrast, anergy is not released in 125Tg/B6/NFATc2â/â B cells following stimulation with anti-CD40. The relief of anergy to BCR stimulation in 125Tg/B6/NFATc2â/â B cells is associated with increased transcription of both NFATc1 and NFATc3 while expression of these NFATs does not change in anti-IgM stimulated 125Tg/B6/NFATc2+/+ B cells. The data suggest that NFATc2 plays a subtle and selective role in maintaining anergy for BCR stimulation by repressing the transcription of other NFAT family members.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 62, Issue 2, December 2014, Pages 321-328
Journal: Molecular Immunology - Volume 62, Issue 2, December 2014, Pages 321-328
نویسندگان
Rachel H. Bonami, William T. Wolfle, James W. Thomas, Peggy L. Kendall,