کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5916927 1163765 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of avian influenza virus NS1 allele on virus replication and innate gene expression in avian cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
The effect of avian influenza virus NS1 allele on virus replication and innate gene expression in avian cells
چکیده انگلیسی


- Both NS1 alleles localize to cytoplasm and nucleus of avian cells.
- Viral replication kinetics was impacted by allele type and avian cell type.
- Avian IFN and IFN inducible genes were examined in response to infection.
- Chimeric NS1 constructs were examined for effect on IFN-β expression.
- Avian cells reveal B allele effects on IFN expression comparable to A allele.

The NS1 gene encoded by Type A influenza virus circulates as two alleles, the A and B allele. The immunomodulatory properties of the NS1 A allele have been thoroughly examined; however, comparisons of allele function have been predominantly made in mammalian systems. Here we show that counter to the current understanding of allele function in mammals, the two alleles similarly regulate elements of the type I interferon (IFN) signaling pathway, including the interferon-inducible genes Mx and 2′-5′ oligoadenylate synthase (2′-5′ OAS), and IL-6, which share the same induction pathway as the interferons in embryo fibroblasts from chickens, turkeys or ducks. Replication of two reassortant viruses demonstrated that the B allele virus replicates more and to higher titers than the A allele virus in duck cells; however, the A allele virus replicates more in the cells from chickens and turkeys. Finally, chimeric constructs were used to identify a region of the NS1 gene that conferred the statistically significant differences in expression and replication observed between the alleles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 56, Issue 4, 31 December 2013, Pages 358-368
نویسندگان
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