کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5918126 | 1163826 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of interleukin-22 in gadoids and examination of its expression level in vaccinated fish
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
This paper reports the cloning and sequencing of interleukin (IL)-22 in two gadoid fish, cod (Gadus morhua) and haddock (Melanogrammus aeglefinus). The complete transcript of this gene was 1002 and 1154Â bp respectively, of which 492Â bp was the open reading frame (ORF) in both genes. High amino acid identity (88.3%) was found between these genes but was less than 50% aa identity to other known genes. The gene organisation of haddock IL-22 consisted of five exons and four introns, as with other IL-10 family members. Expression studies showed that IL-22 is constitutively expressed in gill, with low level expression also observed in gut, gonad and head kidney. In a vaccination experiment, haddock were injected intraperitoneally with formalin-killed Vibrio anguillarum or with PBS, and 2 months later challenged by immersion in 107Â cfu/ml V. anguillarum for 30Â min. Head kidney and gill samples were collected prior to challenge and 24, 48 and 72Â h post-challenge (hpc) for Real-time PCR analysis of IL-22 expression. No significant changes in IL-22 expression were observed in head kidney tissue but vaccinated fish showed a significantly increased expression of IL-22 24hpc in gill and no mortalities were seen in these fish. In contrast, control fish, which started to succumb to the disease from 72hpc, showed no significant increase in gill expression after challenge.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 46, Issue 10, June 2009, Pages 2098-2106
Journal: Molecular Immunology - Volume 46, Issue 10, June 2009, Pages 2098-2106
نویسندگان
Yolanda Corripio-Miyar, Jun Zou, Heather Richmond, Chris J. Secombes,