Prey digestion in the midgut of the predatory bug Podisus nigrispinus (Hemiptera: Pentatomidae)

► The most active salivary proteinase of Podisus nigrispinus is a collagenase. ► Once the collagenase is injected it disrupts the prey tissues that are found inside predator midgut. ► Cathepsins, amylase, aminopeptidase and α-glucosidase were isolated and characterized. ► The midgut digestive enzymes are involved in digesting the pre-orally disrupted prey tissues. ► Carbohydrate is digested in anterior and protein in middle and posterior midgut, respectively.