Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant. ESR analysis of structural changes and their relation with surfactant activity

- Effects of serum, BSA, lipoproteins and cholesterol on dynamic and structural properties of surfactant.
- Serum increases inactive subtype and rigidity in the polar region, consequently inactivates EPS.
- HDL increases structural rigidity and may be responsible for the loss of surface activity.
- There is a correlation between the fluidity in the polar region of PL and the surfactant performance.

The study of structural changes in the surfactant may help to understand the mechanisms by which the surfactant is inactivated by serum. Here, we compared the in vitro effects of serum, albumin, lipoproteins (VLDL, LDL, HDL) and cholesterol on the dynamic and structural properties of surfactant suspensions by electronic spin resonance and surface tension measurements. Our results showed that albumin seems to be responsible for macrostructure disaggregation and increased rigidity in the hydrophobic region, but it did not affect surfactant activity. Fluidity in the polar area seems to be critical for proper physiological activity, and the changes induced by serum observed in this area would be generated by HDL or cholesterol, but the amount of cholesterol transferred by serum is not significant. Statistical analysis showed that surfactant activity correlated with the fluidity in the polar area but not with that in the hydrophobic region. We obtained strong evidence that among all the serum components tested, HDL is the one that causes the structural changes that compromise surfactant performance.