کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5967113 1576163 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The protective effect of GLP-1 analogue in arterial calcification through attenuating osteoblastic differentiation of human VSMCs
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
The protective effect of GLP-1 analogue in arterial calcification through attenuating osteoblastic differentiation of human VSMCs
چکیده انگلیسی


- GLP-1 analogue liraglutide inhibits the osteoblastic differentiation and calcification of human VSMCs.
- PI3K/Akt/mTOR/S6K1 signaling is involved in the role of GLP-1 analogue liraglutide.
- GLP-1 analogues are potential for the treatment of cardiovascular diseases.

BackgroundArterial calcification is a common event in cardiovascular pathogenesis. Osteoblastic differentiation of vascular smooth muscle cells (VSMCs) is the most important cytopathologic foundation of arterial calcification. Glucagon-like peptide-1 (GLP-1) exerts multiple cardioprotective actions beyond insulinotropic effects through GLP-1 receptor (GLP-1R). However, whether GLP-1 regulates osteoblastic differentiation of VSMCs and associated molecular mechanisms has not been clarified.MethodsThe human VSMC differentiation model was established by beta-glycerophosphate (β-GP) induction. The mineralization was measured by Alizarin Red S staining. Protein expression and phosphorylation were detected by Western blot or immunofluorescence. GLP-1R gene expression was silenced by siRNA.ResultsThe GLP-1 analogue liraglutide dose- and time-dependently inhibited the protein expression of osteoblastic differentiation markers alkaline phosphatase (ALP), osteocalcin (OC), and Runt-related transcription factor 2 (Runx2), phosphorylation of PI3K, Akt, mTOR, and S6K1. Silencing of GLP-1R gene expression by siRNA significantly blocked the effects of liraglutide in ALP protein expression and PI3K/Akt phosphorylation.ConclusionGLP-1 analogue liraglutide attenuates the osteoblastic differentiation and calcification of human VSMCs through its receptor and subsequent activation of PI3K/Akt/mTOR/S6K1 signaling. GLP-1 analogues may be potential agents for the treatment of cardiovascular diseases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Cardiology - Volume 189, 15 June 2015, Pages 188-193
نویسندگان
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